R&D Systems Rhesus Macaque TNF-alpha Recombinant Protein
Manufacturer: R&D Systems 1070RM025
DescriptionMeasured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED 50 for this effect is 15-60 pg/mL.
|Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.|
|95%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|<0.01 EU per 1μg of the protein by the LAL method.|
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