R&D Systems TdT Enzyme/Buffer
Purified from E. coli containing a recombinant plasmid harboring the bovine TdT gene
Manufacturer: R&D Systems 951001KEB
Terminal deoxynucleotidyl transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA.
- Enzyme of choice for Trevigen TACS In Situ Apoptosis Detection Kits which utilize TdT to incorporate biotinylated nucleotides into the fragmented DNA associated with apoptosis
- The specificity of TdT for protruding, recessed, blunt-ended dsDNA or for ssDNA is cation dependent
- One unit of enzyme catalyzes the incorporation of 1nmole of dATP into acid-precipitable material in one hour at 37°C under standard assay conditions
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