R&D Systems™ Recombinant Human HMGB1 Protein, CF
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Manufacturer: R&D Systems™ 1690HMB050
DescriptionMeasured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.5-12.5 μg/mL.
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|>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.|
|Met1 & Gly2|
|<0.10 EU / 1 µg of the protein by the LAL method.|
|Reconstitute at 200 µg/mL in PBS.|
|Lyophilized from a 0.2 µm filtered solution in PBS.|
|M.W. (Observed): 28-34 kDa, reducing conditions; M.W. (theoretical): 25 kDa|
|Reconstitute at 200μg/mL in PBS.|
|SDS-PAGE: 28-34kDa, reducing conditions|
|Mouse myeloma cell line, NS0-derived human HMGB1/HMG-1 protein Met1-Glu215|
Safety and Handling
ShelfLife : 365 days
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