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R&D Systems™ Recombinant Human HMGB1 Protein, CF Available on GSA/VA Contract for Federal Government customers only.

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Manufacturer:  R&D Systems™ 1690HMB050

Catalog No. 16-90H-MB050


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Description

Description

Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.5-12.5 μg/mL.

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Specifications

Specifications

HMGB1/HMG-1
Bioactivity
3146
HMGB1/HMG-1
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
50 µg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Met1 & Gly2
<0.10 EU / 1 µg of the protein by the LAL method.
Unconjugated
Reconstitute at 200 µg/mL in PBS.
P09429
Lyophilized from a 0.2 µm filtered solution in PBS.
M.W. (Observed): 28-34 kDa, reducing conditions; M.W. (theoretical): 25 kDa
Reconstitute at 200μg/mL in PBS.
SDS-PAGE: 28-34kDa, reducing conditions
Mouse myeloma cell line, NS0-derived human HMGB1/HMG-1 protein Met1-Glu215
RUO
Carrier Protein
HMGB1
Yes
Safety and Handling

Safety and Handling

ShelfLife : 365 days

SDS
Documents
Product Certifications
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