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R&D Systems™ Recombinant Human HMGB1 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Supplier: R&D Systems™ 1690HMB050
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Description
Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.5-12.5 μg/mL.Specifications
P09429 | |
Lyophilized from a 0.2 μm filtered solution in PBS. | |
M.W. (Observed): 28-34 kDa, reducing conditions; M.W. (theoretical): 25 kDa | |
Reconstitute at 200μg/mL in PBS. | |
50 μg | |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
Met1 & Gly2 | |
<0.10 EU / 1 μg of the protein by the LAL method. | |
Unconjugated | |
Reconstitute at 200 μg/mL in PBS. | |
HMGB1/HMG-1 |
Bioactivity | |
3146 | |
HMGB1/HMG-1 | |
SDS-PAGE: 28-34kDa, reducing conditions | |
Mouse myeloma cell line, NS0-derived human HMGB1/HMG-1 protein Met1-Glu215 | |
RUO | |
Carrier Protein | |
HMGB1 | |
Recombinant | |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
Safety and Handling
ShelfLife : 365 days
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