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RASSF1A Mouse anti-Human, Clone: eB114-10H1, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$119.00 - $305.00

Specifications

Antigen RASSF1A
Clone eB114-10H1
Target Species Human
Conjugate Unconjugated
Isotype IgG1, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-135-58 Non-distribution product as customer accommodation.
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Invitrogen
14688880
25 μg
Each for $119.00
Add to cart
 
50-135-59 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14688882
100 μg
Each for $305.00
Add to cart
 
Description

Description

Description: The eB114 antibody reacts with the RASSF1A isoform of the tumor suppressor gene RASSF1. RASSF1A contains a Ras Association Domain (RA) and binds Ras in a GTP-dependent manner to effect apoptosis. The RASSF1A isoform undergoes epigenetic inactivation in a variety of human primary tumors. This has been associated with hypermethylation of a CpG island(s) in its promoter region. This antibody was generated against a synthetic peptide corresponding to the splice junction region of RASSF1A. Applications Reported: The eB114 antibody has been reported for use in immunoprecipitation, and immunoblotting (WB). Applications Tested: The eB114-10H1 has been tested by Western blotting and can be used at approximately 1-10 µg/mL. RASS1A is detected as a 45 kDa band in Western blotting of a HeLa cell extract. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Specifications

Specifications

RASSF1A
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C
eB114-10H1
Unconjugated
Immunoprecipitation, Western Blot
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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