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Rat anti-Laminin gamma 1, Clone: SPM193, Novus Biologicals™

Rat Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2447520.02MG

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Catalog No. NBP24475200


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Description

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Laminin gamma 1 Monoclonal antibody specifically detects Laminin gamma 1 in Human, Mouse samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.
Specifications

Specifications

Laminin gamma 1
SPM193
Unconjugated
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
LAMB2Laminin-7 subunit gamma, Laminin B2 chain, laminin subunit gamma-1, laminin, gamma 1 (formerly LAMB2), Laminin-1 subunit gamma, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, Laminin-3 subunit gamma, Laminin-4 subunit gamma, Laminin-6 subunit gamma, Laminin-8 subunit gamma, Laminin-9 subunit gamma, MGC87297, S-LAM gamma, S-laminin subunit gamma
Rat
IgG2a Kappa
Protein A or G purified
RUO
Store at 4C.
Monoclonal
Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This MAb reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This MAb stains uniformly all human and murine basement membranes.
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
0.2mg/mL
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Purified
LAMC1
Murine EHS laminin preparation
210 kDa
0.02 mg
Apoptosis, Cancer, Cytoskeleton Markers, Extracellular Matrix, Tumor Suppressors
Primary
3915
Human, Mouse
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