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Rat anti-MHC Class II, Clone: M5/114.15.2, Novus Biologicals™

Rat Monoclonal Antibody

Manufacturer:  Novus Biologicals NBP1433120.5MG

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Catalog No. NBP1433125


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Description

Description

MHC Class II Monoclonal antibody specifically detects MHC Class II in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
Specifications

Specifications

MHC Class II
M5/114.15.2
Unconjugated
Aqueous, may contain carrier protein/stabilizer. with 0.09% Sodium Azide
class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
Rat
IgG2b Kappa
0.5 mg
Adaptive Immunity, Asthma, Cell Biology, Diabetes Research, Immunology, Major Histocompatibility Complexes
Primary
3108
Mouse
Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
0.5 mg/mL
Western Blot 1:100-1:2000, Flow Cytometry < 0.125 ug/10^6 cells, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Affinity Purified
HLA-DMA
The immunogen for this antibody was MHC Class II.
Protein A or G purified
RUO
Store at 4C. Do not freeze.
Monoclonal
The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
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