CD119 Rat anti-Mouse, Brilliant Violet 650, Clone: GR20, BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740498
The GR20 monoclonal antibody specifically binds to CD119, the α chain of the interferon γ(IFN- γ) receptor. This receptor is expressed by mouse leukocytes, endothelial, and epithelial cells, but not by mature erythrocytes. GR20 mAb recognizes an epitope (susceptible to certain fixatives) in the ligand-binding site of the receptor. It can block the binding of mouse IFN- γ; therefore, it can inhibit IFN- γ.mediated effects including the activation of mouse macrophages for tumor cell killing. IFN- γhas been reported to induce the expression of Ly-6A/E antigen on cell lines and normal B lymphocytes, and the GR20 antibody is capable of inhibiting the IFN- γ
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 650|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IgG2a, κ, also known as Brown Norway IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Ifngr1; IFN-γ Receptor α; IFN-gamma R alpha; Ifngr; INF-g Receptor; Cd119|
|BALB/c mouse monomyelocytic cell line WEHI-3|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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