CD13 Rat anti-Mouse, Brilliant Violet 510, Clone: R3-242, BD Optibuild™

Rat Monoclonal Antibody

Supplier:  BD Biosciences 740127

Catalog No. BDB740127

Description

The R3-242 monoclonal antibody specifically binds to aminopeptidase N (APN), which has been reported to be the mouse CD13 homologue. APN is expressed in a variety of mammalian tissues, including intestinal and renal epithelial brush border, endothelium, liver, lung, and brain. In lymphoid tissues, R3-242 mAb is reported to detect APN on monocytes, macrophages, mast cells, dendritic cells, and B lymphocytes, but not on T cells, and thymocytes. However, the reported reactivity with B cells may be non-specific, via binding to the Fc γreceptors (CD16/CD32). This observation is in agreement with reports using different antibodies to mouse APN . APN may play a role in antigen presentation in the immune system. R3-242 mAb does not inhibit the enzymatic activity of APN.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications

• Antigen: CD13
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Anpep; Alanyl aminopeptidase; Aminopeptidase N; AP-N; mAPN; Lap-1
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: R3-242
• Conjugate: Brilliant Violet 510
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Crude Aminopeptidase N Preparation from BALB/c Mouse Intestinal Epithelium
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only