CD13 Rat anti-Mouse, Brilliant Violet 711, Clone: R3-242, BD Optibuild
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740695
The R3-242 monoclonal antibody specifically binds to aminopeptidase N (APN), which has been reported to be the mouse CD13 homologue. APN is expressed in a variety of mammalian tissues, including intestinal and renal epithelial brush border, endothelium, liver, lung, and brain. In lymphoid tissues, R3-242 mAb is reported to detect APN on monocytes, macrophages, mast cells, dendritic cells, and B lymphocytes, but not on T cells, and thymocytes. However, the reported reactivity with B cells may be non-specific, via binding to the Fc γreceptors (CD16/CD32). This observation is in agreement with reports using different antibodies to mouse APN . APN may play a role in antigen presentation in the immune system. R3-242 mAb does not inhibit the enzymatic activity of APN.
The antibody was conjugated to BD Horizon BV711 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy5.5 / Alexa Fluor 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 711|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Anpep; Alanyl aminopeptidase; Aminopeptidase N; AP-N; mAPN; Lap-1|
|Crude Aminopeptidase N Preparation from BALB/c Mouse Intestinal Epithelium|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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