CD16/CD32 Rat anti-Mouse, Brilliant Violet 650, Clone: 2.4G2, BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740457
The 2.4G2 antibody specifically recognizes a common nonpolymorphic epitope on the extracellular domains of the mouse Fc γIII (CD16) and Fc γII (CD32) Receptors. It has also been reported to bind the Fc γI receptor (CD64) via its Fc domain. 2.4G2 mAb blocks non-antigen-specific binding of immunoglobulins to the Fc γIII and Fc γII, and possibly Fc γI, Receptors . CD16 and/or CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 650|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IgG2b, κ, also known as Sprague-Dawley (outbred) IgG2b, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Mouse BALB/c Macrophage J774|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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