CD162 Rat anti-Mouse, BUV395, Clone: 2PH1, BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740273
The 2PH1 monoclonal antibody specifically binds to the N-terminus of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selplg gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. The 2PH1 antibody is reported to block binding of mouse leukocytes to CD62P, but the 4RA10 antibody (Cat. No. 557787) has significantly greater blocking activity.
|Brilliant Ultraviolet 395|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IgG1, κ, also known as Lewis IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Selplg; PSGL-1; Psgl1; Selp1; Selpl; P-selectin glycoprotein ligand 1|
|Ovalbumin-conjugated peptide covering amino acids 42 to 60 of mouse PSGL-1|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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