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CD179b Rat anti-Mouse, Brilliant Violet 650, Clone: LM34, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 740555

Catalog No. BDB740555

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The pre-B cell receptor (pre-BCR) expressed during the early stages of B lymphocyte development is a heterodimer of immunoglobulin heavy chain (IgH) with surrogate light chain, which is an Ig-light-chain-like molecule composed of the non-covalently linked CD179b ( λ 5) and CD179a (VpreB) proteins. The pre-BCR is believed to control IgH repertoire selection and proliferation of differentiating B lymphocytes. The LM34 antibody reacts with λ 5 and surrogate light chain, but not VpreB alone, in transfected X63-Ag8.653 cells. It detects surrogate light chain on pro-B and pre-B cell lines, in the presence or absence of IgH, but not on IgM-positive B lymphocytes. It also detects surrogate light chain or λ 5 on the surface and in the cytoplasm of pro-B or pre-B cells from the bone marrow of normal or RAG2-deficient mice. It has been noted that the (CD45R/B220) cell-surface expression of surrogate light chain is upregulated after a one-hour incubation of bone-marrow leukocytes in tissue culture medium at 37°C. At the earliest stages of B lymphopoiesis, before IgH is available, the surrogate light chain associates with a complex of glycoproteins, including a nonclassical cadherin, which could be involved in selective adhesion events during B-lymphocyte development. After immunization, cell-surface λ 5 is detected on a subset of splenic Ig light chain-positive germinal-center B lymphocytes.



Brilliant Violet 650
Aqueous buffered solution containing ≤0.09% sodium azide.
IgG2a, κ, also known as Lewis IgG2a, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
λ5; Lambda 5; VpreB1; Vpreb-2; Immunoglobulin omega chain; Ig omega
Affinity Purified
and V[preB], Purified soluble complex of recombinant μ IgH, λ5
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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