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CD180 Rat anti-Mouse, Brilliant Violet 650, Clone: RP/14, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 740558

Catalog No. BDB740558

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The RP/14 monoclonal antibody specifically binds to CD180, the “radioprotective” 105 kDa (RP105) antigen expressed on mature B lymphocytes. Like other members of the Toll-like Receptor (TLR) family, the extracellular region of CD180 contains tandem repeats of a leucine-rich motif flanked by regions containing conserved cysteines. These characteristic structural components of TLR may mediate protein-protein interactions and regulation of signal transduction involved in innate immune responses. Furthermore, CD180 associates with the secretory protein MD-1 on the cell surface. Cross-linking of cell-surface CD180 with the RP/14 antibody induces activation signals in B lymphocytes. Despite the expression of CD180 on lymphocytes of all strains tested (BALB/c, BALB/c. xid , C3H, C57BL/6, CBA/N, and 129/Sv), some strains (BALB/c. xid , C57BL/6. xid , CBA/N, 129/Sv, and 129/Ola) display deficient responses to that stimulatory effects of mAb RP/14.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.



Brilliant Violet 650
Aqueous buffered solution containing ≤0.09% sodium azide.
IgG2a, κ, also known as Wistar (outbred) IgG2a, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
Ly78; Ly-78; Lymphocyte antigen 78; RP105; Radioprotective 105 kDa protein
Affinity Purified
BALB/c mouse splenocytes
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Certifications


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