CD180 Rat anti-Mouse, Brilliant Violet 786, Clone: RP/14, BD Optibuild
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740957
The RP/14 monoclonal antibody specifically binds to CD180, the “radioprotective” 105 kDa (RP105) antigen expressed on mature B lymphocytes. Like other members of the Toll-like Receptor (TLR) family, the extracellular region of CD180 contains tandem repeats of a leucine-rich motif flanked by regions containing conserved cysteines. These characteristic structural components of TLR may mediate protein-protein interactions and regulation of signal transduction involved in innate immune responses. Furthermore, CD180 associates with the secretory protein MD-1 on the cell surface. Cross-linking of cell-surface CD180 with the RP/14 antibody induces activation signals in B lymphocytes. Despite the expression of CD180 on lymphocytes of all strains tested (BALB/c, BALB/c. xid , C3H, C57BL/6, CBA/N, and 129/Sv), some strains (BALB/c. xid , C57BL/6. xid , CBA/N, 129/Sv, and 129/Ola) display deficient responses to that stimulatory effects of mAb RP/14.
The antibody was conjugated to BD Horizon BV786 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy7-like dyes (eg, 780/60-nm filter).
|Brilliant Violet 786|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Ly78; Ly-78; Lymphocyte antigen 78; RP105; Radioprotective 105 kDa protein|
|BALB/c mouse splenocytes|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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