CD26 Rat anti-Mouse, Brilliant Violet 421, Clone: H194-112, BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740021
The H194-112 monoclonal antibody specifically binds to CD26, which is also known as, Thymocyte-activating molecule (THAM), or dipeptidyl peptidase IV (DPP IV, Dpp4). CD26 is a ∽220- kDa dimer formed of identical type-II transmembrane core polypeptides which undergo variable post-translational modifications. It is a multi-functional molecule with both ectopeptidase and signal-transducing activities. Studies with specific DPP IV inhibitors suggest that the enzymatic activity is involved in the mediation of T-cell activation events. The expression of CD26 is developmentally regulated in the thymus. Resting lymphoid cells of the bone marrow and peripheral B and T lymphocytes express low levels of CD26; bone-marrow and peritoneal myeloid cells do not. CD26 is also found on epithelial cells in the kidney, liver, small intestine, and lung. Cross-linked H194-112 mAb induces proliferation of immature and mature thymocytes in the presence of either IL-1 plus IL-2 or PMA; addition of IL-2 or IL-4 to PMA further enhances the activation.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
|Brilliant Violet 421|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IgG2a, κ, also known as Louvain, LOU/C, LOU/M IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Cd26; Dpp4; dipeptidylpeptidase 4; THAM, DPP IV|
|Cell-surface molecules solubilized from BALB/c mouse thymocytes|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
For Research Use Only