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CD45RC Rat anti-Mouse, Brilliant Violet 650, Clone: DNL-1.9, BD Optibuild™
Rat Monoclonal Antibody
Supplier: BD Biosciences 740448
Description
The DNL-1.9 antibody monoclonal antibody specifically recognizes an exon C-dependent epitope of CD45 protein which is found at high density on B cells, and at low density on peripheral T cytotoxic/suppressor cells, a subset of peripheral T helper cells, and a very small subset of thymocytes. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOHterminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
CD45RC | |
Monoclonal | |
0.2mg/mL | |
Ptprc; Cd45; CD45R; L-CA; Ly-5; Lyt-4; T200 | |
Rat | |
Affinity Purified | |
RUO | |
Mouse | |
IgG2a κ |
Flow Cytometry | |
DNL-1.9 | |
Brilliant Violet 650 | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
70Z/3 mouse pre-B cell line | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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