CD49d Rat anti-Mouse, Brilliant Violet 421, Clone: 9C10(MFR4.B), BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740016
The 9C10 (MFR4.B) monoclonal antibody specifically binds to the Integrin α 4 chain (CD49d), which is expressed as a heterodimer with either of two β chains, β 1 or β 7. The α 4β 1 integrin (VLA-4, CD49d/CD29) is expressed on most peripheral lymphocytes, thymocytes, and monocytes; while the α β 7 also preferentially binds to the mucosal vascular addressin, MAdCAM-1. Although the 9C10 (MFR4.B) antibody alone has been reported to have little function-blocking activity, it can augment the inhibitory effects of mAb R1-2 (Cat. No. 553153), resulting in almost complete inhibition of VLA-4 binding to VCAM-1.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
|Brilliant Violet 421|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IgG2a, κ, also known as Lewis IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Integrin α4; Itga4; Integrin alpha-4; LPAM alpha; VLA-4 alpha; VLA-4a|
|Mouse (C57BL/6xA/J)F Fetal Liver Mast MC/9|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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