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CD83 Rat anti-Mouse, Brilliant Violet 650, Clone: Michel-19, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 740619

Catalog No. BDB740619


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Description

Description

The Michel-19 antibody reacts with CD83, a member of the immunoglobulin superfamily that is expressed on mature dendritic cells and activated T lymphocytes. Furthermore, thymic cortical epithelial cells express Cd83 transcripts. CD83 is involved in the regulation of T-cell development and immune responses, and its ligand is found on a subpopulation of splenic B lymphocytes.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

Specifications

CD83
Michel-19
Brilliant Violet 650
Affinity Purified
Mouse CD83 Recombinant Protein
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
RUO
Primary
Murine
Flow Cytometry
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Rat
IgG1, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Documents
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