CD45RC Mouse Anti-Rat, Brilliant Violet 510, Clone: OX-22, BD Optibuild
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742487
The OX-22 antibody reacts with CD45RC on pre-B lymphocytes, B cells, CD8+ T suppressor/cytotoxic cells, and a subset of CD4+ T helper (Th) lymphocytes. It weakly reacts with thymocytes. CD45RC is a high-molecular-weight isoform of CD45 (Leukocyte Common Antigen); its level of expression distinguishes subpopulations of CD4+ T cells with Th1-like and Th2-like effector functions. Levels of expression of CD45RC have also been reported to distinguish resting from activated T cells at various stages of maturation. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|PHA-activated rat lymph node cells|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.|
|Brilliant Violet 510|
|Mouse BALB/c IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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