Invitrogen™ IFN gamma Rat ELISA Kit

For the quantitative detection of rat IFN-gamma.

Catalog Number	Mfr. No.	Quantity
50-183-37	Invitrogen™ BMS621	96 Tests
50-183-39	Invitrogen™ BMS621TEN	10 x 96 Tests

Description

The Rat Interferon gamma (Rt IFNγ) ELISA quantitates Rt IFNγ in rat serum or cell culture medium. The assay will exclusively recognize both natural and recombinant rat IFNγ. Principle of the method The Rat IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency. Kits include:

• aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IFN-gamma
• Biotin-Conjugate anti-rat IFN-gamma monoclonal antibody
• Streptavidin-HRP
• Rat IFN-gamma Standard lyophilized, 4ng/mL upon reconstitution
• Sample Diluent
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution (tetramethyl-benzidine)
• Stop Solution (1M Phosphoric acid)
• Blue-Dye, Green-Dye and Red-Dye
• Adhesive Films

Specifications

• Accession Number: P01581
• Assay Sensitivity: 9.9 pg/mL
• Product Type: ELISA Kit
• Target Species: Rat
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IFNG2
• Intraassay CV: 5%
• Label or Dye: HRP
• Sample Volume: Serum, 50 μL; Supernatant, 50 μL
• Synonym: Immune interferon, IFG
• Test Time: 1 hr. 20 min.

• Assay Range: 31.3-2,000 pg/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 25712
• Interassay CV: 10%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Rat
• Total Assay Time: 3 hr. 10 min.

For Research Use Only