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Ig Goat anti-Rat, BB515, Polyclonal, BD

Goat Polyclonal Antibody

Manufacturer:  BD Biosciences 565012

Catalog No. BDB565012


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Description

Description

BD Horizon™ BB515 Goat Anti-Rat Ig is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Rat Ig primary antibodies. It also stains rat B cells and has little reactivity with rat non-B cells or mouse splenocytes. As a second step, it is reactive with rat IgG and IgM monoclonal antibodies although a weaker signal may be detected when the primary antibody has a rat IgM or IgG2b isotype due to its adsorption with mouse Ig. It has weak crossreactivity detectable by flow cytometry with some, but not all, hamster immunoglobulins.

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g. 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

Specifications

Ig
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide
Goat
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Polyclonal
Flow Cytometry
BB515
Affinity purified
Ig
RUO
Primary
Rat
Documents
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