Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ eBioscience™ Rat Ig Isotyping ELISA Ready-SET-Go!™ Kit Non-distribution product as customer accommodation.

Specifically engineered for measurement of rat Immunoglobulins from cell culture supernatants. Up to 12 individual samples can be tested for anti-IgG1, IgG2a, IgG2b, IgG2c, IgA, and IgM as well as light chain kappa and lambda.

Manufacturer:  Invitrogen™ 885064086

 View more versions of this product

Catalog No. 50-112-5159


Add to cart

Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Rat Ig isotype control mixture (positive control), lyophilized
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)

Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Specifications

Specifications

HRP
96-well plates
10X Coating Buffer: Buffer for plating the Capture Antibody
10 x 96 tests
Supernatant
Supernatant,400 μL
2-8°C
24 hr 30 min
HRP
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
50μL
Rat
1 hr 45 min
Rat
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only