Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™, Invitrogen™

Rat Isotype Control

Catalog Number	Mfr. No.	Quantity
50-112-8797	Invitrogen 15432180	25 μg
50-141-13	Invitrogen 15432182	100 μg
50-112-2929	Invitrogen 15432142	100 Tests

Description

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA. Applications Tested: Rat IgG2a Isotype Contrl is offered in 2 formats: - μg size: This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by staining and flow cytometric analysis. This can be used at test size: 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.

Specifications

• Antigen: Rat IgG2a kappa
• Conjugate: PE-Cyanine5
• Host Species: Rat
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography

• Clone: eBR2a
• Form: Liquid
• Regulatory Status: RUO
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.