Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™, Invitrogen™

Rat Isotype Control

Supplier:  Invitrogen 16403181

Catalog No. 50-145-24

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: The monoclonal rat IgG2b kappa isotype control has been reported for use in flow cytometric analysis.Applications Tested: This rat IgG2b isotype control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It can be used at the same concentration as the experimental antibody.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.

Specifications

• Antigen: Rat IgG2b kappa
• Clone: eB149/10H5
• Conjugate: Functional Grade
• Gene Alias: IgG2, IgG2b, IgG2bk
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: None
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay
• Concentration: 1mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C
• Isotype: IgG2b κ

For Research Use Only.