Invitrogen™ Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™, Invitrogen™

Catalog No.	Quantity
50-145-24	50 μg
50-112-9605	500 μg

Catalog No. 50-145-24 Supplier Invitrogen™ Supplier No. 16403181

Description

Rat Isotype Control

Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: The monoclonal rat IgG2b kappa isotype control has been reported for use in flow cytometric analysis. Applications Tested: This rat IgG2b isotype control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It can be used at the same concentration as the experimental antibody. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.

Specifications

• Antigen: Rat IgG2b kappa
• Applications: Control, Flow Cytometry, Functional Assay
• Classification: Monoclonal
• Clone: eB149/10H5
• Concentration: 1 mg/mL
• Conjugate: Functional Grade
• Formulation: PBS with no preservative; pH 7.2
• Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Isotype Control (Primary)
• Target Species: Not Applicable
• Content And Storage: 4°C
• Product Type: Isotype Control
• Form: Liquid
• Isotype: IgG2b κ

Frequently Asked Questions (FAQs)

Is an isotype control a must for flow cytometry analysis?

No. Many users are using unstained cells in combination with FMO controls to identify their positive populations.

What if I can't find the right isotype control for flow cytomtery?

The flow cytometry field is moving away from using isotype controls as they are not necessarily the most appropriate way to control for non-specific binding. Instead, they are using unstained cells to define the negative population, single stained controls to set compensation, and flow minus one (FMO) controls to set regions and gates.

You may want to consider whether using an isotype control is something you need to do. Here are some references you might want to look at:
-O'Gorman MR, Thomas J. (1999) Isotype controls-time to let go? Cytometry 38:78-80.

-Keeney M, Gratama JW, Chin-Yee IH et al. (1998) Isotype controls in the analysis of lymphocytes and CD34+ stem/progenitor cells by flow cytometry- time to let go! Cytometry 34:280-283.

-Hulspas R, O'Gorman MR, Wood BL et al. (2009) Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry B Clin Cytom 76:355–364.

-Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline - Second Edition. Clinical and Laboratory Standards Institute, (CLSI). Document H42-A2 Volume 27 No.16, 2007.

-Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells; Approved Guideline - Second Edition. Clinical and Laboratory Standards Institute, (CLSI). Document H43-A2 Volume 27 No. 11, 2007.

If you do wish to use isotype controls, we offer a wide variety of species and fluorophores, search our Web site under "isotype antibody control."

For Research Use Only.