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  5. Invitrogen™ Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™, Invitrogen™ 

Invitrogen™ Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™, Invitrogen™

Catalog No. 5015561
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Catalog No. 50-155-61 Supplier Invitrogen™ Supplier No. 25403182
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Description

Rat Isotype Control

Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: PE-Cy7 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG2b K Isotype Control PE-Cyanine7 has been tested by flow cytometric analysis of mouse splenocytes. It should be used at the same concentration as the experimental antibody. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Rat IgG2b kappa
Applications Control, Flow Cytometry
Classification Monoclonal
Clone eB149/10H5
Concentration 0.2 mg/mL
Conjugate PE-Cyanine7
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Alias IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Isotype Control (Primary)
Target Species Not Applicable
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Isotype Control
Form Liquid
Isotype IgG2b κ
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For Research Use Only.

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