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Rat IgG2b kappa, Super Bright 702, clone: eB149/10H5, Isotype Control, eBioscience™ Non-distribution product as customer accommodation.

Rat Isotype Control

$150.00 - $312.00

Specifications

Antigen Rat IgG2b kappa
Clone eB149/10H5
Target Species Not Applicable
Conjugate Super Bright 702
Isotype IgG2b, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
67403180 Non-distribution product as customer accommodation. View Documents Invitrogen
67403180
25μg Each for $150.00
N/A
67403182 Non-distribution product as customer accommodation. View Documents Invitrogen
67403182
100μg Each for $312.00
N/A
Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody.Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100µL of cell sample + 100µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser
Specifications

Specifications

Rat IgG2b kappa
Not Applicable
IgG2b, kappa
Liquid
Isotype Control
4°C, store in dark, DO NOT FREEZE!
eB149/10H5
Super Bright 702
Control, Flow Cytometry
Control
PBS with BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only

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