Invitrogen™ Rat IL-9 ELISA Kit

Rat IL-9 quantitates rat IL-9 in serum, plasma, supernatant.

Supplier:  Invitrogen™ ERA34RBX5

Catalog No. ERA34RBX5

Description

Rat IL-9 quantitates rat IL-9 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat IL-9.

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.

Specifications

• Accession Number: cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; Il9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor p40
• Assay Sensitivity: 0.45 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 5 x 96 Tests
• Sample Volume: Supernatant, 50 μL; Plasma, 50 μL; Serum, 50 μL
• Target: IL-9
• Total Assay Time: 4 hr 45 min

• Assay Range: 0.45 to 100 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene Symbol: Il9
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min