Invitrogen™ LIX-Cell Lysate (CXCL5) Rat ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ ERCXCL5CL

Catalog No. ERCXCL5CL

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Rat LIX (CXCL5) ELISA quantitates Rt LIX in rat cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Rt LIX. Principle of the method The Rat LIX solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).

Specifications

• Accession Number: P97885
• Assay Sensitivity: 15 pg/ml
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Cell Lysates
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 60665
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: Rat
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 8.23-6000 pg/ml
• Calibration Range: 15pg/mL to 6000pg/mL
• Conjugate: Biotin
• Protein Family: Chemokine & Receptors
• Specificity: The antibody pair provided in this kit recognizes rat LIX / CXCL5.
• Target Species: Rat
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Cxcl5, Cxcl6
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Cell Lysate,20 μL
• Synonym: Cxcl5
• Test Time: 1 hr. 20 min.