Zyagen Labs Rat mammary, E16 tissue lysate is total protein extracted from healthy Sprague Dawley RAT MAMMARY, E16 tissues in lysis buffer contain protease inhibitors. Ideal for many applications including WB & ELISA. Packed at 2mg/ml or 5mg/ml
Manufacturer: Zyagen Labs RT41416
Zyagen tissue lysate (total protein) is extracted from freshly harvested tissues in protein lysis buffer supplemented with a cocktail of 7 mammalian protease inhibitors to minimize proteolysis. The extracted protein is precisely quantified and packed in 1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one year if stored properly at -80oC. The quality of protein is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody. The extracted protein is ready for immediate use in reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, and protein-protein interaction.
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