Invitrogen™ Rat MIP-1alpha ELISA Kit

Description

ELISA

Rat MIP-1alpha quantitates rat MIP-1alpha in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat MIP-1alpha.

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

Specifications

• Accession Number: AI323804; C-C motif chemokine 3; C-C motif chemokine ligand 3; CCL3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; G0S19-1; Heparin-binding chemotaxis protein; H-MIP-1-alpha; L2G25B; LD78alpha; LD78-alpha; LD78-alpha(4-69); Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1 alpha; MIP1 (a); mip1 alpha; MIP-1 alpha; MIP1-(a); Mip1a; MIP-1 A; MIP-1 alpha; MIP-1-alpha; MIP1-alpha; MIP-1-alpha(4-69); PAT 464.1; RP23-320E6.7; SCYA3; SIS-alpha; SIS-beta; small inducible cytokine A3; small inducible cytokine A3 (homologous to mouse Mip-1 A); small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein; TY-5
• Assay Range: 0.7 to 50 ng/mL
• Assay Sensitivity: 0.7 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 25542
• Gene Symbol: Ccl3
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CCL3 (MIP-1 alpha)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins