Invitrogen™ Rat Notch-1 ELISA Kit

Rat Notch-1 quantitates rat Notch-1 in serum, plasma, supernatant.

Supplier:  Invitrogen™ ERA44RB

Catalog No. ERA44RB

Description

Rat Notch-1 quantitates rat Notch-1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat Notch-1.

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

Specifications

• Accession Number: 9930111A19Rik; AOS5; AOVD1; Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila); hN1; lin-12; major type A protein; Mis6; Motch; Motch A; mT14; N1; Neurogenic locus notch homolog protein 1; NEXT; NICD; NOTCH; Notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch gene homolog 1; Notch homolog 1, translocation-associated; notch receptor 1; NOTCH1; p300; RP23-306D20.12; RP23-306D20.12-001; Tan1; Translocation-associated notch protein TAN-1; transmembrane receptor Notch1
• Assay Sensitivity: 0.09 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Notch1
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min

• Assay Range: 0.09 to 20 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): Q07008
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Supernatant, 50 μL; Plasma, 50 μL; Serum, 50 μL
• Target: NOTCH1
• Total Assay Time: 4 hr 45 min