Invitrogen™ Rat PDGF-BB ELISA Kit

ELISA

Supplier:  Invitrogen™ ERA49RBX10

Catalog No. 50-254-5422

Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Specifications

• Accession Number: Q05028
• Assay Sensitivity: 8 pg/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: PDGFB
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hrs 20 mins

• Assay Range: 8 to 2,000 pg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): 24628
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Target: PDGF-BB
• Total Assay Time: 4 hrs 45 mins