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Invitrogen™ RAGE (AGER) Rat ELISA Kit
Sandwich ELISA Kit
Supplier: Invitrogen™ ERAGER
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.
Description
The Rat RAGE (AGER) ELISA quantitates Rt RAGE in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt RAGE. Principle of the method The Rat RAGE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The receptor for advanced glycation endproducts (RAGE) is a member of the Immunoglobin superfamily of cell surface markers. This protein is able to interact with many molecules including: advanced glycation endproducts (AGE), amphoterin, and ligands. Research has shown that the accumulation of RAGE ligands in the biological system (endothelium mononuclear phagocytes, neurons and smooth muscle cells) can lead to destructive tissue diseases such as diabetic retinopathy, amyloidoses, tumors and inflammation disorders.Specifications
Q63495 | |
300 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
81722 | |
<12% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
96 Tests | |
Development | |
2°C to 8°C | |
Rat | |
4 hr. 45 min. |
0.41-300 ng/mL | |
300pg/mL to 300 ng/ml | |
Biotin | |
Other Proteins | |
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha. | |
Rat | |
Colorimetric Microplate Reader | |
Ager | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL | |
RAGE | |
1 hr. 20 min. |
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