Invitrogen™ Rat VEGFR1 ELISA Kit

Rat VEGFR1 quantitates rat VEGFR1 in serum, plasma, supernatant.

Supplier:  Invitrogen™ ERA59RBX5

Catalog No. ERA59RBX5

Description

Rat VEGFR1 quantitates rat VEGFR1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat VEGFR1.

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR.

Specifications

• Accession Number: AI323757; Embryonic receptor kinase 2; Emrk2; FLT; Flt1; FLT-1; fms related tyrosine kinase 1; FMS-like tyrosine kinase 1; FMS-related tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FRT; i21; sFlt1; soluble VEGFR 1; sVEGFR 1; Tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; Vascular endothelial growth factor receptor 1; vascular endothelial growth factor receptor-1; vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1
• Assay Sensitivity: 0.41 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: FLT1
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min

• Assay Range: 0.41 to 100 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): P53767
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 5 x 96 Tests
• Sample Volume: Supernatant, 50 μL; Plasma, 50 μL; Serum, 50 μL
• Target: VEGF Receptor 1
• Total Assay Time: 4 hr 45 min