Active Motif Recombinant Histone H3K14ac (EPL)
Manufacturer: Active Motif 31254
Recombinant Histone H3 acetyl Lys14 (H3K14ac) proteins are generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a N-terminal histone tail peptide containing acetylated lysine 14 via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. The molecular weight of the recombinant histone is 15,268 Daltons. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest […] For more information, please visit: https://www.activemotif.com/catalog/513.
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