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Novus Biologicals™ Recombinant E. coli Double-Stranded Uracil-DNA Glycosylase Protein

Highly purified and high bioactivity. Generating reliable and reproducible results.

Manufacturer:  Novus Biologicals™ NBP27595450UG

Catalog No. NB002850


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Description

Description

  • A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-168 of E.coli Double-Stranded Uracil-DNA Glycosylase
  • G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family.
  • It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear.
  • The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site.
  • It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base.
  • The complementary strand guanine functions in substrate recognition.
  • Recombinant E.coli mug protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
  • This product is for research use only and is not approved for use in humans or in clinical diagnosis.
Specifications

Specifications

0.5 mg/mL
20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol
>90%, by SDS-PAGE
E. coli
double-strand uracil-DNA glycosylase, Double-strand-specific uracil glycosylase, dsDNA specific UDG, DsUDG, EC 3.2.2.28, G/U mismatch-specific DNA glycosylase, G:T/U mismatch-specific DNA glycosylase, Mismatch-specific uracil DNA glycosylase, Mug, Xanthine DNA glycosylase
SDS-Page
21.1 kDa
50 μg
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol
SDS
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