R&D Systems Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) CF
R&D Systems Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged) CF is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily.
Manufacturer: R&D Systems 2706CL500/CF
DescriptionMeasured in a cell proliferation assay using enriched human B cells in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED 50 for this effect is 2-12 ng/mL in the presence of 10 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ) and 20 ng/mL of recombinant human IL-4.
|Lyophilized from a 0.2μm filtered solution in HEPES, NaCl and EDTA.|
|17.7 kDa (monomer)|
|>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.|
|<0.01 EU per 1μg of the protein by the LAL method.|
|Reconstitute at 100μg/mL in sterile PBS.|
|CD154, CD154 antigen, CD40 antigen ligand, CD40 ligand, CD40L, CD40-L, CD40LG, CD40LIGM, gp39, hCD40L, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution.|