R&D Systems™ Recombinant Human CD40 Ligand/TNFSF5 (Histidine-tagged), CF

A New rh CD40 Ligand is Now Available! ∼10 fold better activity; HEK293 expressed; HA tag!

Supplier:  R&D Systems™ 2706CL500/CF

Catalog No. 27-06C-L500

Description

Measured in a cell proliferation assay using enriched human B cells in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is 2-12 ng/mL in the presence of 10 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ) and 20 ng/mL of recombinant human IL-4.

Specifications

• Accession Number: P29965
• Formulation: Lyophilized from a 0.2 µm filtered solution in HEPES, NaCl and EDTA.
• Molecular Weight (g/mol): M.W. (Observed): 18 kDa, reducing conditions; M.W. (theoretical): 17.7 kDa (monomer)
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Source: E. coli-derived human CD40 Ligand/TNFSF5 protein Glu108-Leu261, with an N-terminal Met and 6-His tag
• Endotoxin Concentration: <0.01 EU / 1 µg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 959
• Name: CD154, CD154 antigen, CD40 antigen ligand, CD40 ligand, CD40L, CD40-L, CD40LG, CD40LIGM, gp39, hCD40L, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5
• Quantity: 500 µg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution.
• Gene Symbol: CD40LG
• Host Species: Human
• Protein: CD40 Ligand/TNFSF5