R&D Systems™ Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.

Supplier:  R&D Systems™ 3718FB100/DUP

Catalog No. 37-18F-B100

Description

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.

Specifications

• Accession Number: NP_001997
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
• Molecular Weight (g/mol): M.W. (theoretical): 16 kDa
• Quantity: 100 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Gene Symbol: FGF2
• Species: Human
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 2247
• Name: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
• Source: E. coli-derived human FGF basic/FGF2/bFGF protein Ala144-Ser288
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Endotoxin Level: <0.01 EU per 1 μg of the protein by the LAL method.
• Protein: FGF basic/FGF2/bFGF