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R&D Systems™ Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.

Manufacturer:  R&D Systems™ 3718FB100

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Catalog No. 37-18F-B100


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Description

Description

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL. The specific activity of Recombinant Human FGF basic/FGF2/bFGF (Catalog # 3718-FB) is approximately 3.1 x 106 IU/mg, which is calibrated against human FGF basic/FGF2/bFGF WHO International Standard (NIBSC code: 90/712). Specific activity is for reference purposes only and is not routinely tested.
Specifications

Specifications

FGF basic/FGF2/bFGF
NP_001997
Bioactivity
2247
Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
100 µg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
FGF2
Reconstitute at 100 µg/mL in sterile PBS.
Human
<0.01 EU per 1 µg of the protein by the LAL method.
Lyophilized from a 0.2 µm filtered solution in Tris-HCl and NaCl.
M.W. (theoretical): 16 kDa
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
E. coli-derived human FGF basic/FGF2/bFGF protein Ala144-Ser288
<0.01 EU / 1 µg of the protein by the LAL method.
Unconjugated
Lyophilized from a 0.2 µm filtered solution in MOPS, Na2SO4, EDTA and DTT.
SDS
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