R&D Systems Recombinant Human HGF (NS0-expressed) Protein, CF
Human Recombinant Protein
Manufacturer: R&D Systems 294HGN250/CF
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 0.1-0.5 ng/mL.
The specific activity of recombinant human HGF is approximately 1.5 x 103 IU/µg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564).
Source: Mouse myeloma cell line, NS0-derived human HGF protein
|Lyophilized from a 0.2 μm filtered solution in PBS.|
|alpha chain: 53.7 kDa beta chain: 26 kDa|
|Manual defrost freezer; Avoid repeated freeze-thaw cycles; 12 mo. from receipt, -20 to -70°C as supplied; 1 mo, 2 to 8°C under sterile conditions after reconstitution; 3 mo, -20 to -70°C under sterile conditions after reconstitution|
|<0.10 EU per 1 μg of the protein by the LAL method.|
|>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Mouse myeloma cell line, NS0-derived human HGF protein Gln32-Ser728|
|Reconstitute at 100 μg/mL in sterile PBS.|
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