R&D Systems™ Recombinant Human IL-10 (aa 19-178) Protein, CF

Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is 0.15 to 0.75 ng/mL.

Supplier:  R&D Systems™ 1064IL025/CF

Catalog No. 10-64I-L025CF

Description

Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.075-0.750 ng/mL.

Specifications

• Accession Number: P22301
• Gene ID (Entrez): 3586
• Name: CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF
• Source: E. coli-derived human IL-10 protein Ser19-Asn178, with an N-terminal Met
• Gene Symbol: IL10
• Species: Human
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): 18.8 kDa
• Quantity: 25 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.