R&D Systems™ Recombinant Human IL-6 (HEK293-expressed) Protein

Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. ED50 for this effect is 0.1 to 0.6 ng/mL

Supplier:  R&D Systems™ 7270IL100/CF

Catalog No. 72-70I-L100CF

Description

Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.1-0.6 ng/mL.

Specifications

• Accession Number: P05231
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 22 kDa, reducing conditions; M.W. (theoretical): 20.8 kDa
• Quantity: 100 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Gene Alias: B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
• Protein: IL-6

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3569
• Name: B cell stimulatory factor-2, B-cell differentiation factor, BSF2, BSF-2, BSF2CTL differentiation factor, CDF, HGFHSFIFNB2Hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6B-cell stimulatory factor 2, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A
• Source: Human embryonic kidney cell, HEK293-derived human IL-6 protein Met1-Met212
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: IL6
• Species: Human
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.