R&D Systems™ Recombinant Human MICB His-tag Protein

Description

Measured by its binding ability in a functional ELISA.

When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK ) is immobilized at 0.125 μg/mL, 100 μL/well, the concentration of Recombinant Human MICB His-tag (Catalog # 10431-MB) that produces 50% of the optimal binding response is approximately 80-400 ng/mL.

Specifications

• Accession Number: NP_005922.2
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 4277
• Molecular Weight (g/mol): Observed: 35-55 kDa, under reducing conditions; Theroretical: 33 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human MICB protein Ala23-Thr308, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Protein: MICB