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R&D Systems™ Recombinant Human/Mouse/Rat BMP-2 (E. coli-expressed), CF

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. ED50 for this effect is 0.08 to 0.48 µg/mL.

Manufacturer:  R&D Systems™ 355BEC025/CF

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Catalog No. 35-5BE-C025CF


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Description

Description

Specifications

Specifications

Human, Mouse, Rat
P12643
650
BDA2, BMP2, BMP-2, BMP-2A, BMP2ABone morphogenetic protein 2A, bone morphogenetic protein 2, SSFSC
25 µg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Unconjugated
Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.
<0.01 EU per 1 µg of the protein by the LAL method.
Bioactivity
12.8 kDa (monomer)
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
E. coli-derived BMP-2 protein Ala284-Arg396 This form is not detected using human BMP-2 Quantikine ELISA (Catalog # DBP200 ).
BMP2
Reconstitute at 100 µg/mL in sterile 4 mM HCl.
SDS
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