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R&D Systems™ Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein

R&D Systems™ Recombinant Human/Mouse/Rat/Canine/Equine BDNF Protein offers 100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined.

Manufacturer:  R&D Systems™ 248BD250/CF

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Catalog No. 24-8BD-250CF


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Description

Description

Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED 50 for this effect is 0.2-2 ng/mL. The specific activity of Recombinant Human/Mouse/Rat/Canine/Equine BDNF is approximately 1.3 x 10 3 units/µg, which is calibrated against recombinant human BDNF WHO Standard (NIBSC code: 96/534). Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera (Catalog # 688-TK ) is coated at 1 µg/mL, Recombinant Human BDNF binds with an apparent K d <1 nM.
Specifications

Specifications

P23560
Bioactivity
627
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
abrineurin, ANON2, brain-derived neurotrophic factor, BULN2,mgC34632, neurotrophin
Recombinant Protein
Human, Mouse, Rat, Canine, Equine
Bioactivity
Lyophilized from a 0.2μm filtered solution in Sodium Citrate and NaCl.
13.5kDa
250μg
<0.10EU per 1μg of the protein by the LAL method.
BDNF
Unconjugated
Reconstitute at 100μg/mL in sterile PBS.
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