R&D Systems™ Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 2 to 10 ng/mL.

Supplier:  R&D Systems™ 788G8100

Catalog No. 78-8G8-100

Specifications

• Accession Number: O08689
• Gene ID (Entrez): 2660
• Name: GDF8, GDF-8, GDF8growth differentiation factor 8, growth/differentiation factor 8, MSLHP, MSTN, Myostatin
• Source: Mouse myeloma cell line, NS0-derived GDF-8/Myostatin protein Asp268-Ser376
• Gene Symbol: MSTN
• Species: Human, Mouse, Rat
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.

• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): 12.4 kDa
• Quantity: 100 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.