R&D Systems™ Recombinant Human Notum Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human Notum Protein is derived from CHO. The Recombinant Human Notum Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q6P988
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 147111
• Molecular Weight (g/mol): Observed M.W.: 38-45kDa, reducing conditions, Theoretical M.W.: 43kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line,CHO-derived human Notum protein Ser81-Thr451 (Cys330Ser) with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: EC 3.-, notum pectinacetylesterase homolog (Drosophila), protein notum homolog
• Common Name: NOTUM
• Biological Activity: Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.1-0.6μg/mL in the presence of 0.5μg/mL of Recombinant Human Wnt-3a.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Ser81-Thr451 (Cys330Ser) with a C-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining