R&D Systems™ Recombinant Human PDGF-AA, Animal-Free Protein
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. ED50 for this effect is 50 to 200 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin.
Manufacturer: R&D Systems™ AFL221050
|<0.01 EU per 1 µg of the protein by the LAL method.|
|>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|E. coli-derived human PDGF-AA protein Ser87-Thr211, with and without an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.|
|Reconstitute at 0.2 mg/mL in sterile 4 mM HCl.|
|M.W. theroretical: 14 kDa (monomer)|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.|
|Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.|
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