R&D Systems™ Recombinant Human R-Spondin 1 Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Supplier:  R&D Systems™ 4645RS100

Catalog No. 46-45RS-100

Description

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.00-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).

Specifications

• Accession Number: Q2MKA7
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Molecular Weight (g/mol): 25.6 kDa
• Source: Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein Ser21-Ala263
• Regulatory Status: RUO
• Gene Symbol: RSPO1
• Cross Reactivity: R-Spondin 1
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 284654
• Quantity: 100 μg
• Storage Requirements: Manual defrost freezer; Avoid repeated freeze-thaw cycles; 12 mo. from receipt, -20 to -70°C as supplied; 1 mo, 2 to 8°C under sterile conditions after reconstitution; 3 mo, -20 to -70°C under sterile conditions after reconstitution
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Species: Human
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.