R&D Systems™ Recombinant Human SIRP alpha/CD172a mFc Chimera Protein, CF

Mouse IgG2a Fc Tag

Supplier:  R&D Systems™ 10651SA050

Catalog No. 10651SA050

Description

Measured by its binding ability in a functional ELISA. When Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD ) is immobilized at 0.1 μg/mL (100 μL/well), Recombinant Human SIRP alpha/CD172a Mouse IgG2a Fc Chimera (Catalog # 10651-SA) binds with an ED50 of 5-40 ng/mL.

Specifications

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 140885
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Source: Chinese Hamster Ovary cell line, CHO-derived human SIRP alpha/CD172a protein Human SIRP alpha/CD172a (Gly27-Arg370) & (Glu31-Arg370) (N-terminus) Accession # NP_542970.1 IEGRMDP Mouse IgG2a (Glu98-Lys330) (C-terminus)
• Endotoxin Concentration: <0.10 EU / 1 µg of the protein by the LAL method.
• Conjugate: Unconjugated
• Protein: SIRP alpha/CD172a

• Formulation: Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose.
• Molecular Weight (g/mol): M.W.-observed: 86-97 kDa, under reducing conditions, M.W.-theroretical: 65 kDa
• Quantity: 50 µg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Gene Alias: Bit, BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MFRtyrosine phosphatase SHP substrate 1, MYD1, MYD-1, MyD-1 antigen, P84, protein tyrosine phosphatase, non-receptor type substrate 1, PTPNS1, SHP substrate 1, SHPS1, SHPS-1, SHPS1CD172A, signal-regulatory protein alpha, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, SIRPA, SIRPalpha, SIRP-ALPHA-1, Sirp-alpha-2, SIRPalpha2, Sirp-alpha-3, SIRPtyrosine-protein phosphatase non-receptor type substrate 1
• Reconstitution: Reconstitute at 500 µg/mL in PBS.